Mouse Embryonic Fibroblast (MEF) Conditioned Media
R&D Systems, part of Bio-Techne | Catalog # AR005
Key Product Details
Species
Mouse
Product Summary for Mouse Embryonic Fibroblast (MEF) Conditioned Media
Kit Summary
Conditioned media to support growth and maintain the pluripotency of embryonic and induced pluripotent stem cells.
Key Benefits
- Eliminates the need for a MEF feeder layer
- Lot-to-lot consistency decreases experimental variability
- Addition of FGF basic generates complete media
Why Culture Stem Cells Using MEF Conditioned Media?
Feeder layers of mouse embryonic fibroblasts (MEF) support stem cell growth and the maintenance of pluripotency by secreting growth factors, cytokines, and nutrients. However, the use of MEFs as a feeder layer can be laborious and MEF contamination is a significant concern.
MEF conditioned media represents an efficient alternative to MEF feeder cells and is one of the mostly widely used feeder-free systems for embryonic stem cell culture. MEF Conditioned Media includes the soluble factors required to support stem cell growth and pluripotency.
Mouse Embryonic Fibroblast (MEF) Conditioned Media:
- Widely used feeder-free system for culturing embryonic and induced pluripotent stem cells.
- Lot-to-lot consistency decreases experimental variability.
- Eliminates the need for a MEF feeder layer.
- Free of mycoplasma and microbial contamination.
- Generate complete media by adding FGF basic (4ng/mL) (Catalog # 233-FB or 4114-TC).
Scientific Data Images for Mouse Embryonic Fibroblast (MEF) Conditioned Media
Induced Pluripotent Stem Cells Grown in MEF Conditioned Media Express Pluripotent Stem Cell Markers SSEA-4, Oct-3/4, Oct-4A, and E-Cadherin.
Induced Pluripotent Stem Cells Grown in MEF Conditioned Media Express Pluripotent Stem Cell Markers SSEA-4. Oct-3/4, Oct-4A, and E-Cadherin.Human iPS2 stem cells were cultured in MEF Conditioned Media (Catalog # AR005) supplemented with 4 ng/mL Recombinant Human FGF basic (Catalog # 233-FBor Catalog # 4114-TC).A. Expression of SSEA-4 was detected using a Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435) followed by a NorthernLights™ (NL) 493-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL009, green). Expression of Oct-3/4 was detected using a Goat Anti-Human Oct-3/4 Antigen Affinity Purified Polyclonal Antibody (Catalog # AF1759) followed by NL557-conjugated Donkey Anti-Goat IgG (Catalog # NL001, red). The nuclei were counterstained with DAPI.B. Expression of Oct-4A was detected using Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) followed by a NorthernLights™ 493-conjugated Donkey Anti-mouse Polyclonal Antibody (Catalog # NL009, green). Expression of E-Cadherin was detected using a Goat Anti-Human E-Cadherin Antigen Affinity Purified Polyclonal Antibody (Catalog # AF648) followed by a NL557-conjugated Donkey Anti-Goat IgG Secondary Antibody (Catalog # NL001, red). The nuclei were counterstained with DAPI. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Human Emryonic Fibroblast Conditioned Media Express Pluripotent Markers SSEA-4 and Oct-3/4.
Human Emryonic Fibroblast Conditioned Media Express Pluripotent Markers SSEA-4 and Oct-3/4. A.Human embryonic stem cells were cultured in Mouse Embryonic Fibroblast (MEF) Conditioned Media (Catalog # AR005) supplemented with 4 ng/mL Recombinant Human FGF basic (Catalog # 233-FB). SSEA-4 and Oct-3/4 were detected using a Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, red) and a Goat Anti-Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759, green). The nuclei were counterstained with DAPI (blue).B.Human embryonic stem cells were cultured in MEF Condition Media supplemented with 4 ng/mL Recombinant Human FGF basic. SSEA-4 and Oct-3/4 were detected as described above. The nuclei were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Embryonic Stem Cells
Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.
R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.
Additional Embryonic Stem Cells Products
Product Documents for Mouse Embryonic Fibroblast (MEF) Conditioned Media
Product Specific Notices for Mouse Embryonic Fibroblast (MEF) Conditioned Media
For research use only
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