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ITS Media Supplement (100X) Best Seller

R&D Systems, part of Bio-Techne | Catalog # AR013

Insulin-Transferrin-Selenium Media Supplement
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AR013

Key Product Details

Species

Multi-Species

Product Summary for ITS Media Supplement (100X)

Kit Summary

Supports cell proliferation under low serum conditions.

Key Benefits

  • Contains optimized concentrations of insulin, transferrin, and selenite
  • Chemically-defined media decreases experimental variation
  • High lot-to-lot consistency decreases variation
  • Performance tested on multiple cell lines

Why Use Pre-optimized and Serum-free Supplements for Cell Culture?

The efficiency of cell proliferation and maintenance of cell health can vary from one experiment to another depending on the media components, the supplements used to drive proliferation, and the quality of the starting cell population.

Although commonly used, serum-containing medium is inherently variable due to the undefined composition and lot-to-lot variability across batches of serum. Troubleshooting media variability can be a costly and time-consuming obstacle that can be minimized through the use of high quality media. Our ITS Media Supplement is a pre-optimized and serum-free media supplement that was designed to help maintain culture media consistency across passages and experiments. The ITS Media Supplement contains optimized concentrations of human insulin, human transferrin, and selenite.

ITS Media Supplement (100x):

  • Contains optimized concentrations of insulin, transferrin, and selenite
  • Chemically-defined media decreases experimental variation
  • High lot-to-lot consistency decreases variation
  • QC-tested on multiple cell lines

Kit Contents

5 mL ITS Media Supplement (100x)

Component Amount (µg/mL)
Recombinant Human Insulin 1000
Human Transferrin 500
Selenite 0.67

Stability and Storage

Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.

Precautions

This product contains human transferrin. This transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. 

Limitations

  • FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
  • The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
  • This reagent should not be used beyond the expiration date indicated on the label.

Scientific Data Images for ITS Media Supplement (100X)

ITS Media Supplement Supports Proliferation Under Low Serum Conditions.

ITS Media Supplement Supports Proliferation Under Low Serum Conditions.

ITS Media Supplement Supports Proliferation Under Low Serum Conditions.Hela, MCF7, and HepG2 cells were cultured in media supplemented with 10% FBS, low serum (0.1% FBS), or ITS Media Supplement + 0.1% FBS. Cells were trypsinized, harvested, and cell counts were performed. For each respective cell line, cell counts were normalized against standard 10% FBS-containing media (100%).

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for ITS Media Supplement (100X)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for ITS Media Supplement (100X)

For research use only

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