Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
Catalog # 3533-010-02 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Features: | Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME ),Type 2 is specifically qualified to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoids. |
Key Benefits: |
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Citations for Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear (44)
Citations are publications that use Bio-Techne products. Selected citations for Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear include:
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Species: Mouse
Sample Types: Organoid
Applications: BioassayBhin et al. (2023-05-19), Multi-omics analysis reveals distinct non-reversion mechanisms of PARPi resistance in BRCA1- versus BRCA2-deficient mammary tumors Cell reports
PMID: 37209095 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayWit et al. (2023-05-19), A histone deacetylase 3 and mitochondrial complex I axis regulates toxic formaldehyde production Science advances
PMID: 37196082 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayMV Luna Velez et al. (2023-02-22), ONECUT2 regulates RANKL-dependent enterocyte and microfold cell differentiation in the small intestine; a multi-omics study Nucleic Acids Research, 2023-00(0).
PMID: 36625255 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Brundu et al. (2023-02-01), Mutated axon guidance gene PLXNB2 sustains growth and invasiveness of stem cells isolated from cancers of unknown primary Embo Molecular Medicine, 2023-00(0):e16104.
PMID: 36722641 -
Species: Human
Sample Types: Whole Tissue
Applications: BioassayIH de Lange et al. (2023-01-12), Hypoxia-Driven Changes in a Human Intestinal Organoid Model and the Protective Effects of Hydrolyzed Whey Nutrients, 2023-015(2).
PMID: 36678267 -
Species: Human
Sample Types: Organoids
Applications: BioassayAL Caipa Garc et al. (2022-12-29), Metabolic Activation of Benzo[a]pyrene by Human Tissue Organoid Cultures International Journal of Molecular Sciences, 2022-124(1).
PMID: 36614051 -
Species: Human
Sample Types: Organoids
Applications: BioassayASN Ng et al. (2022-12-13), AKTIP loss is enriched in ERalpha-positive breast cancer for tumorigenesis and confers endocrine resistance Cell Reports, 2022-141(11):111821.
PMID: 36516775 -
Species: Mouse
Sample Types: Oganoids
Applications: BioassayA Cañellas-S et al. (2022-11-09), Metastatic recurrence in colorectal cancer arises from residual EMP1+ cells Nature, 2022-1611(7936):603-613.
PMID: 36352230 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJE van Leeuwe et al. (2022-10-24), Computational pharmacogenomic screen identifies drugs that potentiate the anti-breast cancer activity of statins Nature Communications, 2022-113(1):6323.
PMID: 36280687 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayA Spanjaard et al. (2022-07-22), Division of labor within the DNA damage tolerance system reveals non-epistatic and clinically actionable targets for precision cancer medicine Nucleic Acids Research, 2022-050(13):7420-7435.
PMID: 35819193 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayZ Diamantopo et al. (2022-06-22), The metastatic spread of breast cancer accelerates during sleep Nature, 2022-0607(7917):156-162.
PMID: 35732738 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayAE Hall et al. (2022-05-19), RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer Nature Communications, 2022-013(1):2791.
PMID: 35589755 -
Species: Human
Sample Types: Organoids
FJM Roos et al. (2022-05-05), Human branching cholangiocyte organoids recapitulate functional bile duct formation Cell Stem Cell, 2022-029(5):776-794.e13.
PMID: 35523140 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayK Shah et al. (2022-04-05), Cell-intrinsic Aryl Hydrocarbon Receptor signalling is required for the resolution of injury-induced colonic stem cells Nature Communications, 2022-013(1):1827.
PMID: 35383166 -
Species: Human
Sample Types: Organoids
Applications: BioassayYY Chen et al. (2022-02-10), Copy number amplification of ENSA promotes the progression of triple-negative breast cancer via cholesterol biosynthesis Nature Communications, 2022-013(1):791.
PMID: 35145111 -
Species: Human
Sample Types: Organoids
Applications: BioassayY Xiao et al. (2022-02-01), Comprehensive metabolomics expands precision medicine for triple-negative breast cancer Cell Research, 2022-00(0).
PMID: 35105939 -
Species: Human
Sample Types: Organoids
Applications: BioassayY Bollen et al. (2022-01-28), Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage PloS Biology, 2022-020(1):e3001527.
PMID: 35089911 -
Species: Human
Sample Types: Organoids
Applications: BioassayBH Choi et al. (2022-01-18), Lineage-specific silencing of PSAT1 induces serine auxotrophy and sensitivity to dietary serine starvation in luminal breast tumors Cell Reports, 2022-038(3):110278.
PMID: 35045283 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayBM Tran et al. (2022-01-13), Air-Liquid-Interface Differentiated Human Nose Epithelium: A Robust Primary Tissue Culture Model of SARS-CoV-2 Infection International Journal of Molecular Sciences, 2022-023(2).
PMID: 35055020 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayJ van der Va et al. (2021-12-21), Adult mouse and human organoids derived from thyroid follicular cells and modeling of Graves' hyperthyroidism Proceedings of the National Academy of Sciences of the United States of America, 2021-1118(51).
PMID: 34916298 -
Species: Human
Sample Types: Whole Cells
C Mathieu et al. (2021-09-12), A Bioluminescent 3CLPro Activity Assay to Monitor SARS-CoV-2 Replication and Identify Inhibitors Viruses, 2021-013(9).
PMID: 34578395 -
Species: Human
Sample Types: Organoids
Applications: BioassayC Calandrini et al. (2021-08-24), Organoid-based drug screening reveals neddylation as therapeutic target for malignant rhabdoid tumors Cell Reports, 2021-036(8):109568.
PMID: 34433038 -
Species: Human
Sample Types: Organoid
Applications: Cell CultureL Signati et al. (2021-08-10), Ultrastructural analysis of breast cancer patient-derived organoids Cancer Cell International, 2021-021(1):423.
PMID: 34376194 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayF De Bacco et al. (2021-07-27), ERBB3 overexpression due to miR-205 inactivation confers sensitivity to FGF, metabolic activation, and liability to ERBB3 targeting in glioblastoma Cell Reports, 2021-036(4):109455.
PMID: 34320350 -
Species: Mouse
Sample Types: Organoid
V Gudiño et al. (2021-04-20), RAC1B modulates intestinal tumourigenesis via modulation of WNT and EGFR signalling pathways Nature Communications, 2021-012(1):2335.
PMID: 33879799 -
Species: Human
Sample Types: Organoid
Applications: BioassayL Gijzen et al. (2021-03-05), Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip Nature Protocols, 2021-00(0).
PMID: 33674788 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayM Yang et al. (2021-01-28), Inhibition of mitochondrial function by metformin increases glucose uptake, glycolysis and GDF-15 release from intestinal cells Scientific Reports, 2021-011(1):2529.
PMID: 33510216 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayDY Tulasi et al. (2021-01-23), Sox9EGFP defines biliary epithelial heterogeneity downstream of Yap activity Cellular and Molecular Gastroenterology and Hepatology, 2021-00(0).
PMID: 33497866 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureS Yasuda et al. (2020-11-16), Loss of sphingosine 1-phosphate receptor 3 gene function impairs injury-induced stromal angiogenesis in mouse cornea Lab Invest, 2020-10(0).
PMID: 33199821 -
Species: Human
Sample Types: Organoid
LC Demmers et al. (2020-10-21), Single-cell derived tumor organoids display diversity in HLA class I peptide presentation Nat Commun, 2020-111(1):5338.
PMID: 33087703 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayC Donato et al. (2020-09-08), Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 2020-032(10):108105.
PMID: 32905777 -
Species: Mouse
Sample Types: Organoid
Applications: BioassayP Francica et al. (2020-08-25), Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining Cell Rep, 2020-032(8):108068.
PMID: 32846126 -
Species: Human
Sample Types: Organoid
E Driehuis et al. (2020-05-18), Patient-derived oral mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemia PLoS ONE, 2020-015(5):e0231588.
PMID: 32421698 -
Species: Mouse
Sample Types: Whole Cells
Applications: Cell CultureZ Kahounová et al. (2020-05-12), Slug-expressing mouse prostate epithelial cells have increased stem cell potential Stem Cell Res, 2020-046(0):101844.
PMID: 32590255 -
Species: Human
Sample Types: Organoid
Applications: BioassayJM Rosenbluth et al. (2020-04-06), Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages Nat Commun, 2020-011(1):1711.
PMID: 32249764 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureC Calandrini et al. (2020-03-11), An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity Nat Commun, 2020-011(1):1310.
PMID: 32161258 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayE Driehuis et al. (2019-12-09), Pancreatic cancer organoids recapitulate disease and allow personalized drug screening Proc. Natl. Acad. Sci. U.S.A., 2019-10(0).
PMID: 31818951 -
Species: Human
Sample Types: Organoid
E Driehuis et al. (2019-05-03), Oral mucosal organoids as a potential platform for personalized cancer therapy Cancer Discov, 2019-00(0).
PMID: 31053628 -
Species: Mouse
Sample Types: Organoid
P Antas et al. (2019-02-26), SH3BP4 Regulates Intestinal Stem Cells and Tumorigenesis by Modulating ?-Catenin Nuclear Localization Cell Rep, 2019-026(9):2266-2273.e4.
PMID: 30811977 -
Species: Mouse
Sample Types: Transfected Whole Cells
Applications: BioassayBM Szczerba et al. (2019-02-06), Neutrophils escort circulating tumour cells to enable cell cycle progression Nature, 2019-00(0).
PMID: 30728496 -
Sample Types: Organoids
Applications: BioassayJ Fakhiri et al. (2019-01-18), Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses Mol Ther Methods Clin Dev, 2019-012(0):202-222.
PMID: 30766894 -
Species: Human
Sample Types: Whole Cells
DM Brix et al. (2019-01-08), Release of transcriptional repression via ErbB2-induced, SUMO-directed phosphorylation of myeloid zinc finger-1 serine 27 activates lysosome redistribution and invasion Oncogene, 2019-00(0).
PMID: 30622337 -
Species: Human
Sample Types: Organoid
Applications: BioassaySachs N et al. (2017-12-07), A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. Cell, 2017-1172(1):373-386.e10.
PMID: 29224780 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayCibin T Raghavan et al. (2016-06-04), AGE-RAGE interaction in the TGF?2-mediated epithelial to mesenchymal transition of human lens epithelial cells. Glycoconjugate Journal, 2016-00(0):1573-4986.
PMID: 27263094
There are no citations that match your criteria.
Customer Reviews (5)
Customer Images
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Verified Customer | Posted 06/18/2022dissolve it on ice, and don't put it outside for too not time
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Verified Customer | Posted 02/21/2022Aortic sprouting assay: mouse aortic ring cultured in a 48-well plate and imaged in an inverted microscope after 5 days. Results are very consistent with this BME (used one layer under and another on top of the aortic ring).
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Verified Customer | Posted 04/13/2021
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Verified Customer | Posted 12/09/2020We are using the BME at a final dilution of 1/30 for the maintenance of iPSC and differentiation into endoderm as well as mesoderm lineages. Attachment, growth, and differentiation of our iPSC is consistent, and we observed only minor variations between different lots.
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Application: Cell migration/motilityReason for Rating: it can form a very nice tube in our control group within 6-8 hour.Verified Customer | Posted 06/01/2018
There are no reviews that match your criteria.
FAQs
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What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
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How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
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What is the Tube Formation Assay?
The Tube Formation Assay is based on the ability of endothelial cells to form three-dimensional capillary-like tubular structures when cultured on a hydrogel of reconstituted basement membrane, such as Cultrex Basement Membrane Extract (BME).
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What are the advantages of the Tube Formation Assay?
The Tube Formation Assay is the most widely used in vitro angiogenesis assay. The assay is rapid, inexpensive and quantifiable. It can be used to identify potentially angiogenic and anti-angiogenic factors, to determine endothelial cell phenotype, and to study pathways and mechanisms involved in angiogenesis. It can be performed in a high throughput mode to screen for a large number of compounds.
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What cell types can be used in the Tube Formation Assay?
The Tube Formation Assay is specific for endothelial cells, either primary cells or immortalized cell lines. Only endothelial cells form capillary-like structures with a lumen inside. Other endothelial cell types form other structures.
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What are the variables associated with the Tube Formation Assay?
The major variables associated with tube formation are composition of the Cultrex Basement Membrane Extract (BME) hydrogel, thickness of the hydrogel, cell density, composition of angiogenic factors in the assay medium, and assay period.
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Which Cultrex Basement Membrane Extract (BME) should I use for the Tube Formation Assay?
Cultrex Reduced Growth Factor BME (RGF BME) is generally used for testing compounds that promote angiogenesis because formation of capillary-like structures (tubes) is significantly less compared to non-growth factor reduced varieties of Cultrex BME. The Cultrex In Vitro Angiogeneis Assay (Tube Formation) includes a qualified production lot of Cultrex RGF BME that exhibits reduced background tube formation in the absence of angiogenic factors.
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How do I reduce spontaneous formation of tubular structures on Cultrex BME in the absence of angiogenic factors?
Primary endothelial cells, such as Human Umbilical Vein Endothelial Cells (HUVECs) form capillary-like structures in the absence of added angiogenic factors less often than immortalized endothelial cells. Generally, reducing the number of cells per cm2 plated onto Cultrex BME will result in less background or spontaneous tube formation. Titrate the number of cells and find optimal conditions for your specific cell line. When endothelial cells fully form capillary structures in response to angiogenic activators, but not in their absence, you may proceed.
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Does Cultrex BME, Catalog # 3533-005-02, affect fluorescence readings when Alamar Blue is used for final readout of assay?
BME is known to have autofluorescence, but if appropriate controls are evaluated, background can be successfully subtracted. A BME only control well with no cells should be used to subtract the background fluorescence.