Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear

Catalog # 3533-010-02 | R&D Systems, Inc. a Bio-Techne Brand
For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

Key Product Details

Features: Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME ),Type 2 is specifically qualified to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoids.
Key Benefits:
  • Qualified for use in organoid cell culture
  • Commonly used robust and established organoid systems
  • Reduced growth factor formulation provides a more defined culture system
  • Quality controlled for performance consistency
Mouse Intestinal Organoids Cultured in Cultrex RGF BME Type 2_3533-005-02
(6)
Human Lung Organoids Cultured in Cultrex RGF BME, Type 2
Liver Organoid Formation and Differentiation in Cultrex RGF BME
Human Intestinal Organoids Cultured using Cultrex RGF BME
HepG2 Spheroids in Cultrex RGF BME, Type 2
Cultrex RGF Basement Membrane Extract, Type 2
Catalog #
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3533-001-02
3533-010-02
3533-005-02
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Setting the standard in quality research reagents
  • Preparation & Storage

    • Preparation & Storage

    Stability Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
    Shipping Conditions The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
    Storage Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • Citations
  • Reviews
  • FAQs

    • Citations for Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear (44)

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    Customer Reviews (5)

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    • Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
      Name: Anonymous
      Verified Customer | Posted 06/18/2022
      dissolve it on ice, and don't put it outside for too not time
      Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear 3533-010-02
    • Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
      Name: Anonymous
      Verified Customer | Posted 02/21/2022
      Aortic sprouting assay: mouse aortic ring cultured in a 48-well plate and imaged in an inverted microscope after 5 days. Results are very consistent with this BME (used one layer under and another on top of the aortic ring).
      Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear 3533-010-02
    • Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
      Name: Anonymous
      Verified Customer | Posted 04/13/2021
    • Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear
      Name: Anonymous
      Verified Customer | Posted 12/09/2020
      We are using the BME at a final dilution of 1/30 for the maintenance of iPSC and differentiation into endoderm as well as mesoderm lineages. Attachment, growth, and differentiation of our iPSC is consistent, and we observed only minor variations between different lots.
    • Cultrex PathClear Reduced Growth Factor BME (2 x 5 mL)
      Name: wenyi wu
      Application: Cell migration/motility
      Reason for Rating: it can form a very nice tube in our control group within 6-8 hour.
      Verified Customer | Posted 06/01/2018
      Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear Cell migration/motility 3533-010-02

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    FAQs

    • What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

      Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

    • How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

      All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

    • How should Cultrex Basement Membrane Extract (BME) be stored and handled?

      Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

    • What is the Tube Formation Assay?

      The Tube Formation Assay is based on the ability of endothelial cells to form three-dimensional capillary-like tubular structures when cultured on a hydrogel of reconstituted basement membrane, such as Cultrex Basement Membrane Extract (BME).

    • What are the advantages of the Tube Formation Assay?

      The Tube Formation Assay is the most widely used in vitro angiogenesis assay. The assay is rapid, inexpensive and quantifiable. It can be used to identify potentially angiogenic and anti-angiogenic factors, to determine endothelial cell phenotype, and to study pathways and mechanisms involved in angiogenesis. It can be performed in a high throughput mode to screen for a large number of compounds.

    • What cell types can be used in the Tube Formation Assay?

      The Tube Formation Assay is specific for endothelial cells, either primary cells or immortalized cell lines. Only endothelial cells form capillary-like structures with a lumen inside. Other endothelial cell types form other structures.

    • What are the variables associated with the Tube Formation Assay?

      The major variables associated with tube formation are composition of the Cultrex Basement Membrane Extract (BME) hydrogel, thickness of the hydrogel, cell density, composition of angiogenic factors in the assay medium, and assay period.

    • Which Cultrex Basement Membrane Extract (BME) should I use for the Tube Formation Assay?

      Cultrex Reduced Growth Factor BME (RGF BME) is generally used for testing compounds that promote angiogenesis because formation of capillary-like structures (tubes) is significantly less compared to non-growth factor reduced varieties of Cultrex BME. The Cultrex In Vitro Angiogeneis Assay (Tube Formation) includes a qualified production lot of Cultrex RGF BME that exhibits reduced background tube formation in the absence of angiogenic factors.

    • How do I reduce spontaneous formation of tubular structures on Cultrex BME in the absence of angiogenic factors?

      Primary endothelial cells, such as Human Umbilical Vein Endothelial Cells (HUVECs) form capillary-like structures in the absence of added angiogenic factors less often than immortalized endothelial cells. Generally, reducing the number of cells per cm2 plated onto Cultrex BME will result in less background or spontaneous tube formation. Titrate the number of cells and find optimal conditions for your specific cell line. When endothelial cells fully form capillary structures in response to angiogenic activators, but not in their absence, you may proceed.

    • Does Cultrex BME, Catalog # 3533-005-02, affect fluorescence readings when Alamar Blue is used for final readout of assay? 

      BME is known to have autofluorescence, but if appropriate controls are evaluated, background can be successfully subtracted. A BME only control well with no cells should be used to subtract the background fluorescence.

    Product Documents

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