CryoDefend-Cell Lines (5 x 10 mL)
R&D Systems, part of Bio-Techne | Catalog # CCM019
Media for defined, protein-free cryopreservation of your cultured cells.
Key Product Details
Species
Multi-Species
Product Summary for CryoDefend-Cell Lines (5 x 10 mL)
CryoDefend-Cell Lines Media
Media for defined, protein-free cryopreservation of cultured cells.
Key Benefits
- Reduce experimental variability by using fully defined media
- Supports post-thaw cell viability better than conventional freezing media
- Optimized for cryopreservation of cultured cells
- Suitable for all cell types
Why use fully defined, protein-free media to cryopreserve cells?
Cryopreservation can be used to maintain cells at a low passage number and to reduce the risk of contamination by microbes and other cell types. The viability of the recovered cells depends on cell density, the type of cryoprotectant used, the method of cooling, and the amount of the cryoprotectant that remains in the culture after the cells have been thawed.
R&D Systems offers CryoDefend® Cell Lines Media, a defined, protein-free media, which has been optimized for the cryopreservation of cultured cells. This specially formulated media contains a defined serum substitute as well as an optimized concentration of a cryopreservative that increases the recovery and viability of healthy cells compared to conventional freezing media.
Kit Contents
Components
- 5 x 10 mL vials of CryoDefend® Cell Lines Media
Scientific Data Images for CryoDefend-Cell Lines (5 x 10 mL)
Cell Viability Following Cryopreservation in CryoDefend®Cell Lines Media in Comparison to Conventional Cryopreservation Media.
A variety of cell lines were frozen in triplicate in either control freezing media (90% culture media/10% DMSO) or CryoDefend®Cell Lines (CryoDefend®-CL) media at 1 x 106cells/cryovial and stored in liquid nitrogen for one week. The cells were then thawed, counted (blue bars), resuspended in standard culture media, and plated into T75 flasks. After 3-5 days in culture, the cells were harvested and counted (red bars). Error bars shown indicate the standard deviation.Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for CryoDefend-Cell Lines (5 x 10 mL)
Product Specific Notices for CryoDefend-Cell Lines (5 x 10 mL)
For research use only
Loading...
Loading...
Loading...
Loading...