Proteome Profiler Human XL Oncology Array Best Seller

Kit Summary

To detect differences in 84 cancer-related proteins between samples. No specialized equipment is necessary.

• Troubleshooting Guide
• Compatible with LI-COR* and chemiluminescence detection

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, tissue lysates, serum, plasma, urine, saliva, or human milk are diluted and incubated with the Human XL Oncology Array overnight. The array is washed to remove unbound proteins, followed by incubation with a cocktail of biotinylated detection antibodies. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2^° C to 8^°.