*If using a LI-COR, additional reagents and protocol modifications are required. Refer to protocol.
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, tissue lysates, serum, plasma, urine, saliva, or human milk are diluted and incubated with the Human XL Oncology Array overnight. The array is washed to remove unbound proteins, followed by incubation with a cocktail of biotinylated detection antibodies. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
4 Array Membranes
Detection Antibody Cocktail
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.
Simultaneously detect the levels of these cancer-related proteins in a single sample.
CD25/IL-2 R alpha
Product Specifications for Proteome Profiler Human XL Oncology Array
Preparation & Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Refer to the product datasheet for complete product details.
Briefly, relative expression levels of human cancer-related proteins in samples can be determined using the following procedure:
Prepare membrane and incubate with prepared sample
Incubate the membrane with Detection Antibody Cocktail
Incubate the membrane array with Streptavidin-HRP
Develop the membrane array with Chemi Reagents 1 and 2
FAQs for Proteome Profiler Human XL Oncology Array
Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
Product Documents for Proteome Profiler Human XL Oncology Array