ZNF544 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89224
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HQRTHTGEKPYECNLCGKSFSQSSKLITHQRIHTGEKPYQCIECGKSFRWNSNLVIHQRIHTGEKPYDCTHCGKSFSQSYQLVAHKRTHTGEKPYECNECGKAFNRSTQLIRHLQIHTGEKPYKCNQCNKA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZNF544 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ZNF544 Antibody [NBP1-89224]](https://resources.bio-techne.com/images/products/ZNF544-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89224-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ZNF544 Antibody [NBP1-89224]")
Immunocytochemistry/ Immunofluorescence: ZNF544 Antibody [NBP1-89224]
Immunocytochemistry/Immunofluorescence: ZNF544 Antibody [NBP1-89224] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.![Immunohistochemistry-Paraffin: ZNF544 Antibody [NBP1-89224]](https://resources.bio-techne.com/images/products/ZNF544-Antibody-Immunohistochemistry-Paraffin-NBP1-89224-img0002.jpg "Immunohistochemistry-Paraffin: ZNF544 Antibody [NBP1-89224]")
Immunohistochemistry-Paraffin: ZNF544 Antibody [NBP1-89224]
Immunohistochemistry-Paraffin: ZNF544 Antibody [NBP1-89224] - Staining of human liver shows strong nuclear positivity in hepatocytes and bile duct cells.
Chromatin Immunoprecipitation ChIP: ZNF544 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-ZNF544 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for ZNF544 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF544
Alternate Names
AF020591, zinc finger protein 544
Gene Symbol
ZNF544
Additional ZNF544 Products
Product Documents for ZNF544 Antibody - BSA Free
Product Specific Notices for ZNF544 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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