Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14850
Discontinued Product
NBP3-14850 has been discontinued.
View all Zika Virus VLP products.
Key Product Details
Species Reactivity
Virus
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # IG3-1-A12-G9
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant Zika virus VLPs (comprising envelope, pre-membrane and membrane proteins), produced in HEK293 cells
Reactivity Notes
Zika
Specificity
This antibody (isotype IgG1 kappa) recognizes the Zika virus VLP comprising Envelope, Pre-membrane and Membrane proteins.
Antibody also recognises Envelope proteins from Dengue virus, Japanese Encephalitis virus and West Nile virus in ELISA assay.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Addition of 0.09% w/v sodium azide is recommended for long term storage at +4C.
Scientific Data Images for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free
Western Blot: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]
Western Blot: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - 100ng of each antigen was used for SDS-PAGE, in non-reduced form. Proteins were transferred using Transblot for 7 minutes at 25V. 5% dry milk in PBS-T was used as blocking buffer and dilution buffer for antibodies. Primary antibodies are given below, and goat anti-mouse-IgG-HRP secondary antibody was used at 1:1000. All steps were carried out for 1h at room temperature with gentle rocking. KPL Membrane TMB was used for detection. Development time 30 seconds.ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]
ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antigen down ELISA using Zika virus VLPs. Antibody also recognises Dengue virus and Chikungunya virus VLPs.ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]
ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antibody recognises Zika virus envelope protein and envelope proteins from Dengue virus (produced in insect and mammalian cells), Japanese Encephalitis virus and West Nile virus.Applications for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Suitable for use in direct ELISA and Western blot (non-reduced).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Zika Virus VLP
Alternate Names
polyprotein, partial
Product Documents for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free
Product Specific Notices for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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