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Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14850

Novus Biologicals, part of Bio-Techne
Discontinued Product
NBP3-14850 has been discontinued. View all Zika Virus VLP products.

Key Product Details

Species Reactivity

Virus

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # IG3-1-A12-G9

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant Zika virus VLPs (comprising envelope, pre-membrane and membrane proteins), produced in HEK293 cells

Reactivity Notes

Zika

Specificity

This antibody (isotype IgG1 kappa) recognizes the Zika virus VLP comprising Envelope, Pre-membrane and Membrane proteins. Antibody also recognises Envelope proteins from Dengue virus, Japanese Encephalitis virus and West Nile virus in ELISA assay.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Addition of 0.09% w/v sodium azide is recommended for long term storage at +4C.

Scientific Data Images for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free

Western Blot: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]

Western Blot: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]

Western Blot: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - 100ng of each antigen was used for SDS-PAGE, in non-reduced form. Proteins were transferred using Transblot for 7 minutes at 25V. 5% dry milk in PBS-T was used as blocking buffer and dilution buffer for antibodies. Primary antibodies are given below, and goat anti-mouse-IgG-HRP secondary antibody was used at 1:1000. All steps were carried out for 1h at room temperature with gentle rocking. KPL Membrane TMB was used for detection. Development time 30 seconds.
ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]

ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]

ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antigen down ELISA using Zika virus VLPs. Antibody also recognises Dengue virus and Chikungunya virus VLPs.
ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]

ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850]

ELISA: Zika Virus VLP Antibody (IG3-1-A12-G9) [NBP3-14850] - Antibody recognises Zika virus envelope protein and envelope proteins from Dengue virus (produced in insect and mammalian cells), Japanese Encephalitis virus and West Nile virus.

Applications for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Suitable for use in direct ELISA and Western blot (non-reduced).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Zika Virus VLP

Alternate Names

polyprotein, partial

Product Documents for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Zika Virus VLP Antibody (IG3-1-A12-G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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