Zika virus (H/PF/2013) NS1 Antibody (GT5212) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13589
Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT5212
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus (H/PF/2013) NS1 (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.
Reactivity Notes
Zika virus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Zika virus (H/PF/2013) NS1 Antibody (GT5212) - Azide and BSA Free
Western Blot: Zika virus (H/PF/2013) NS1 Antibody (GT5212) [NBP3-13589]
Western Blot: Zika virus (H/PF/2013) NS1 Antibody (GT5212) [NBP3-13589] - Non-infected (-) and infected (+) C6/36 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus (H/PF/2013) NS1 antibody [GT5212] (NBP3-13589) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Zika virus (H/PF/2013) NS1 Antibody (GT5212) [NBP3-13589]
Immunocytochemistry/Immunofluorescence: Zika virus (H/PF/2013) NS1 Antibody (GT5212) [NBP3-13589] - Immunofluorescent analysis of non-infected and infected vero cells using Zika virus (H/PF/2013) NS1 antibody [GT5212] (NBP3-13589). Green: Zika virus (H/PF/2013) NS1 antibody [GT5212] (NBP3-13589) diluted at 1:500.Western Blot: Zika virus (H/PF/2013) NS1 Antibody (GT5212) [NBP3-13589]
Western Blot: Zika virus (H/PF/2013) NS1 Antibody (GT5212) [NBP3-13589] - Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus (H/PF/2013) NS1 antibody [GT5212] (NBP3-13589) diluted at 1:2500.Applications for Zika virus (H/PF/2013) NS1 Antibody (GT5212) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:1000-1:10000
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Sandwich ELISA
Assay dependent
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Zika Virus (H/PF/2013) NS1
Long Name
Zika Virus Polyprotein
Additional Zika Virus (H/PF/2013) NS1 Products
Product Documents for Zika virus (H/PF/2013) NS1 Antibody (GT5212) - Azide and BSA Free
Product Specific Notices for Zika virus (H/PF/2013) NS1 Antibody (GT5212) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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