Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846]

Immunocytochemistry/Immunofluorescence: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846] - Vero cells were seeded on coverslips and infected with Yellow Fever 17D virus for 24 h at MOI 2. Control coverslips consisted of uninfected cells. After fixation with 4% PFA, samples were stained with the antibodies. Antibody was used at a dilution of 1:1000 dilution and Triton X-100 was used as detergent. Imaging was performed using a Leica SP5 confocal microscope (Image by Virology Research Services Ltd).

Direct ELISA: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846] - Assay showing specificity of Yellow Fever Virus NS1 antibody for Yellow Fever Virus NS1. NS1 antigens from different flaviviruses were coated on an ELISA plate at 0.5ug/ml, and detected at 0.01ug/ml, visualized with Goat anti-mouse IgG:HRP.

Sandwich ELISA: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846] - ELISA plates coated with Mouse Anti-Yellow Fever Virus NS1 antibody were used to capture YFV NS1 protein followed by detection with biotinylated Yellow Fever Virus NS1 antibody.