YAF2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89444

Novus Biologicals, part of Bio-Techne
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NBP1-89444
NBP1-89444-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all YAF2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AFKCMMCDVRKGTSTRKPRPVSQLVAQQVTQQFVPPTQSKKEKKDKVEKEKSEKETTSKKNSHKKTRPRLKNVDRSSAQHLEVTVGDLTVIITDFKEKTKSPPASSAASADQHSQSGSSSDNTERGMSRSSS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for YAF2 Antibody - BSA Free

YAF2 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: YAF2 Antibody - BSA Free [NBP1-89444]

Immunocytochemistry/ Immunofluorescence: YAF2 Antibody - BSA Free [NBP1-89444]

Staining of human cell line U-2 OS shows localization to nucleoplasm.
YAF2 Antibody - BSA Free Immunohistochemistry-Paraffin: YAF2 Antibody - BSA Free [NBP1-89444]

Immunohistochemistry-Paraffin: YAF2 Antibody - BSA Free [NBP1-89444]

Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
YAF2 Antibody - BSA Free Immunohistochemistry-Paraffin: YAF2 Antibody - BSA Free [NBP1-89444]

Immunohistochemistry-Paraffin: YAF2 Antibody - BSA Free [NBP1-89444]

Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
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Applications for YAF2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: YAF2

The protein encoded by the YAF2 gene interacts with YY1, a zinc finger protein involved in negative regulation ofmuscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrastto YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by thecalcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effectof YY1. (provided by RefSeq)

Alternate Names

DKFZp779H1820, MGC41856, YY1 associated factor 2, YY1-associated factor 2

Gene Symbol

YAF2

Additional YAF2 Products

Product Documents for YAF2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for YAF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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