Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all WWP1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 200 and 250 of human WW Domain Containing E3 Ubiquitin Protein Ligase 1 using the numbering given in entry NP_008944.1 (GeneID 11059).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WWP1 Antibody - BSA Free

Western Blot: WWP1 Antibody [NBP1-49712]

Western Blot: WWP1 Antibody [NBP1-49712]

Western Blot: WWP1 Antibody [NBP1-49712] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WWP1 antibody NBP1-49712 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WWP1 was also immunoprecipitated by rabbit anti-WWP1 antibody NBP1-49713, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for WWP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WWP1

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.

Long Name

WW Domain Containing E3 Ubiquitin Protein Ligase 1

Alternate Names

AIP5, HSDRP1, Tiul11

Gene Symbol

WWP1

Additional WWP1 Products

Product Documents for WWP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WWP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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