Wnt-3a Antibody (HL1911) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25753
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1911 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Wnt-3a.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Wnt-3a Antibody (HL1911) - Azide and BSA Free (NBP3-25753) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Wnt-3a Antibody (HL1911) - Azide and BSA Free
Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -
Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -
Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] -
Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Applications for Wnt-3a Antibody (HL1911) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-3a
Long Name
Wingless-type MMTV Integration Site Family, Member 3a
Alternate Names
Wnt3a
Gene Symbol
WNT3A
Additional Wnt-3a Products
Product Documents for Wnt-3a Antibody (HL1911) - Azide and BSA Free
Product Specific Notices for Wnt-3a Antibody (HL1911) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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