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VGLUT2 Antibody (CL2921)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52463

Recombinant Monoclonal Antibody
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NBP3-52463

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL2921

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9P2U8, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VGLUT2 Antibody (CL2921)

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of human cerebellum shows moderate to strong positivity in glutamatergic fibers.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP3-52463]

Staining of mouse brain shows positivity in glutamatergic fibers in the cerebellum.

Applications for VGLUT2 Antibody (CL2921)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGLUT2

VGLUT2 is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity.

Alternate Names

differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2

Gene Symbol

SLC17A6

Additional VGLUT2 Products

Product Documents for VGLUT2 Antibody (CL2921)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VGLUT2 Antibody (CL2921)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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