VARS Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05970
![Western Blot: VARS Antibody [NBP3-05970]](https://resources.bio-techne.com/images/products/VARS-Antibody-Western-Blot-NBP3-05970-img0001.jpg "Western Blot: VARS Antibody [NBP3-05970]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human VARS fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for VARS Antibody - BSA Free
Western Blot: VARS Antibody [NBP3-05970]
Western Blot: VARS Antibody [NBP3-05970] - Lane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:140 kDaObserved band size:140 kDa(We are unsure as to the identity of these extra bands.)![Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970]](https://resources.bio-techne.com/images/products/VARS-Antibody-Immunohistochemistry-Paraffin-NBP3-05970-img0002.jpg "Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970]")
Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970]
Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970] - Staining of human VARS in human brain with rabbit polyclonal antibody at 1:500 dilution.![Immunoprecipitation: VARS Antibody [NBP3-05970]](https://resources.bio-techne.com/images/products/VARS-Antibody-Immunoprecipitation-NBP3-05970-img0004.jpg "Immunoprecipitation: VARS Antibody [NBP3-05970]")
Immunoprecipitation: VARS Antibody [NBP3-05970]
Immunoprecipitation: VARS Antibody [NBP3-05970] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-VARS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-VARS rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 140 kDaObserved band size :140 kDaApplications for VARS Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:200-1:2000
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VARS
Alternate Names
EC 6.1.1, EC 6.1.1.9, G7AVARS1, Protein G7a, valine tRNA ligase 1, cytoplasmic, Valine--tRNA ligase, ValRS, valyl-tRNA synthetase, valyl-tRNA synthetase 2, VARS2valRS
Gene Symbol
VARS1
Additional VARS Products
Product Specific Notices for VARS Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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