UQCRC2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38078
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2).
Sequence:
GVSHPVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFLEEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGNLGHTPFVDEL
Sequence:
GVSHPVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFLEEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGNLGHTPFVDEL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit UQCRC2 Antibody - BSA Free (NBP3-38078) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for UQCRC2 Antibody - BSA Free
Immunoprecipitation: UQCRC2 Antibody [NBP3-38078] -
Immunoprecipitation: UQCRC2 Antibody [NBP3-38078] - Immunoprecipitation analysis of 200 ug extracts of HepG2 cells using UQCRC2 antibody. Western blot was performed from the immunoprecipitate using UQCRC2 antibody at a dilution of 1:1000.
Immunohistochemistry: UQCRC2 Antibody [NBP3-38078] -
Immunohistochemistry: UQCRC2 Antibody [NBP3-38078] - Immunohistochemistry analysis of paraffin-embedded Human colon using UQCRC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Western Blot: UQCRC2 Antibody [NBP3-38078] -
Western Blot: UQCRC2 Antibody [NBP3-38078] - Western blot analysis of various lysates using UQCRC2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for UQCRC2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50 - 1:200
Immunoprecipitation
0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UQCRC2
Alternate Names
Complex III subunit 2, Core protein II, cytochrome b-c1 complex subunit 2, mitochondrial, QCR2, ubiquinol-cytochrome c reductase core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCR2
Gene Symbol
UQCRC2
Additional UQCRC2 Products
Product Documents for UQCRC2 Antibody - BSA Free
Product Specific Notices for UQCRC2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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