UQCRC2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20816
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Primate (99%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human UQCRC2. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (99%), Mouse (82%), Rat (81%), Bovine (88%).
Localization
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit UQCRC2 Antibody (NBP2-20816) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for UQCRC2 Antibody
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP2-20816]](https://resources.bio-techne.com/images/products/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP2-20816-img0006.jpg "Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP2-20816]")
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP2-20816]
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP2-20816] - Paraffin-embedded breast cancer. UQCRC2 antibody dilution: 1:250.![Western Blot: UQCRC2 Antibody [NBP2-20816]](https://resources.bio-techne.com/images/products/UQCRC2-Antibody-Western-Blot-NBP2-20816-img0007.jpg "Western Blot: UQCRC2 Antibody [NBP2-20816]")
Western Blot: UQCRC2 Antibody [NBP2-20816]
Western Blot: UQCRC2 Antibody [NBP2-20816] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation: UQCRC2 Antibody [NBP2-20816] -
UQCRC2 antibody immunoprecipitates UQCRC2 protein in IP experiments.IP samples: HepG2 whole cell extract
A. 35 ug HepG2 whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of UQCRC2 protein by 4 ug UQCRC2 antibody (NBP2-20816)
10 % SDS-PAGE
The immunoprecipitated UQCRC2 protein was detected by UQCRC2 antibody (NBP2-20816) diluted at 1:5000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Applications for UQCRC2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:5000-1:20000
Application Notes
Western Blot (WB) Assay-dependent dilution
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: UQCRC2
Alternate Names
Complex III subunit 2, Core protein II, cytochrome b-c1 complex subunit 2, mitochondrial, QCR2, ubiquinol-cytochrome c reductase core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCR2
Gene Symbol
UQCRC2
UniProt
Additional UQCRC2 Products
Product Documents for UQCRC2 Antibody
Product Specific Notices for UQCRC2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.