ULK1 Antibody (24GB2195)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50661

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NBP3-50661-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB2195

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ULK1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ULK1 (Uniprot: O75385)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ULK1 Antibody (24GB2195)

ULK1 Antibody (24GB2195)

Immunocytochemistry/ Immunofluorescence: ULK1 Antibody (24GB2195)[NBP3-50661] -

Immunocytochemical staining of HepG2 cells with ULK1 antibody (1:1,000). Nuclei were stained blue with DAPI; ULK1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
ULK1 Antibody (24GB2195)

Flow Cytometry: ULK1 Antibody (24GB2195)[NBP3-50661] -

Flow cytometric analysis of ULK1expression in HepG2 cells using ULK1 antibody (1:2,000). Green, isotype control; red, ULK1.
ULK1 Antibody (24GB2195)

Western Blot: ULK1 Antibody (24GB2195)[NBP3-50661] -

Western blotting analysis using ULK1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for ULK1 Antibody (24GB2195)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ULK1

ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Long Name

Serine/threonine-protein kinase ULK1

Alternate Names

ATG1, hATG1, KIAA0722

Gene Symbol

ULK1

Additional ULK1 Products

Product Documents for ULK1 Antibody (24GB2195)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ULK1 Antibody (24GB2195)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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