UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43791

Novus Biologicals, part of Bio-Techne
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NBP3-43791
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Catalog #
BSA Free
NBP2-46621
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3210

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P09936, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free (NBP2-46621) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free

UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line U-251 AAb
UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]

Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Mouse Cerebral Cortex tissue
View 7 more images

Applications for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Gene Symbol

UCHL1

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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