UBE2R1/CDC34 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84946
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit UBE2R1/CDC34 Antibody - BSA Free (NBP1-84946) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for UBE2R1/CDC34 Antibody - BSA Free
Western Blot: UBE2R1/CDC34 Antibody [NBP1-84946]
Western Blot: UbcH3/Cdc34 Antibody [NBP1-84946] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-112Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-84946]
Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-84946] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol.Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody [NBP1-84946]
Immunohistochemistry-Paraffin: UbcH3/Cdc34 Antibody [NBP1-84946] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.Applications for UBE2R1/CDC34 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UBE2R1/CDC34
Alternate Names
Cdc34, UBC3
Gene Symbol
CDC34
Additional UBE2R1/CDC34 Products
Product Documents for UBE2R1/CDC34 Antibody - BSA Free
Product Specific Notices for UBE2R1/CDC34 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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