UbcH7/UBE2L3 Antibody (24GB1875)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51593
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB1875
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human UbcH7/UBE2L3 (Uniprot: P68036)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UbcH7/UBE2L3 Antibody (24GB1875)
Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody (24GB1875)[NBP3-51593] -
Immunocytochemical staining of HepG2 cells with UbcH7/UBE2L3 antibody (1:1,000). Nuclei were stained blue with DAPI; UbcH7/UBE2L3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: UbcH7/UBE2L3 Antibody (24GB1875)[NBP3-51593] -
Validation of UbcH7/UBE2L3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with UbcH7/UBE2L3 antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: UbcH7/UBE2L3 Antibody (24GB1875)[NBP3-51593] -
Flow cytometric analysis of UbcH7/UBE2L3expression in HepG2 cells using UbcH7/UBE2L3 antibody (1:2,000). Green, isotype control; red, UBE2L3.Applications for UbcH7/UBE2L3 Antibody (24GB1875)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UbcH7/UBE2L3
Alternate Names
E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3
Gene Symbol
UBE2L3
Additional UbcH7/UBE2L3 Products
Product Documents for UbcH7/UBE2L3 Antibody (24GB1875)
Product Specific Notices for UbcH7/UBE2L3 Antibody (24GB1875)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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