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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S74

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7

Localization

Membrane

Specificity

Detects approx 220kDa. No cross-reactivity against TrpM6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TRPM7 Antibody (S74) - BSA Free

Western Blot: TRPM7 Antibody (S74) [NBP2-12906]

Western Blot: TRPM7 Antibody (S74) [NBP2-12906]

Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906]

Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906]

Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpM7 Antibody (D) Composite.
Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906]

Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906]

Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Applications for TRPM7 Antibody (S74) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of TrpM7 Antibody was sufficient for detection of TrpM7 in 10 ug of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPM7

1 ug/mL was sufficient for detection of TrpM7 in 10ug of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Alternate Names

CHAK, CHAK1FLJ25718, Channel-kinase 1, EC 2.7.11.1, Long transient receptor potential channel 7, LTRPC ion channel family member 7, LTrpC7, LTrpC-7, LTRPC7FLJ20117, transient receptor potential cation channel subfamily M member 7, transient receptor potential cation channel, subfamily M, member 7, transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK

Gene Symbol

TRPM7

Additional TRPM7 Products

Product Documents for TRPM7 Antibody (S74) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRPM7 Antibody (S74) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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