TRPC3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32258

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TRPC3 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 275 to 325 of human Transient Receptor Potential Cation Channel, Subfamily C, Member 3 using the numbering given in entry Q13507.3 (GeneID 7222).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TRPC3 Antibody - BSA Free (NBP2-32258) is a polyclonal antibody validated for use in WB and IP. Anti-TRPC3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRPC3 Antibody - BSA Free

Western Blot: TRPC3 Antibody [NBP2-32258]

Western Blot: TRPC3 Antibody [NBP2-32258]

Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TRPC3 Antibody [NBP2-32258]

Immunoprecipitation: TRPC3 Antibody [NBP2-32258]

Immunoprecipitation: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for IP at 6 ug per reaction. TRPC3 was also immunoprecipitated by rabbit anti-TRPC3 antibody BL16089. For blotting immunoprecipitated TRPC3, NBP2-32258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for TRPC3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRPC3

The transient receptor potential canonical (TRPC) group of channels, including TPRC3, is a family of receptor-activated non-selective calcium permeant cation channels. In vitro TRPC proteins can form hetero- or homo-oligomeric channels. Endogenous TRPC1, TRPC3 and TRPC7 participate in forming heteromeric store-operated channels, while TRPC3 and TRPC7 can also participate in forming heteromeric receptor-operated channels. TRPC3 plays important roles in neuronal differentiation and immune cell maturation by mediating the cationic current in response to phospholipase C activation, Ca(2+) depletion, and diacylglycerol stimulation. TRPC3 channels are highly expressed in pontine neurons and mediate a nonselective cation current in response to the neurotrophin receptor, TrkB.

Long Name

Short transient receptor potential channel 3

Alternate Names

hTrp-3, hTrp3, TRP-3, TRP3

Gene Symbol

TRPC3

UniProt

Q13507.3 (Human)

Additional TRPC3 Products

Product Documents for TRPC3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRPC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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