TOE1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04022
![Immunoprecipitation: TOE1 Antibody [NBP2-04022]](https://resources.bio-techne.com/images/products/TOE1-Antibody-Immunoprecipitation-NBP2-04022-img0001.jpg "Immunoprecipitation: TOE1 Antibody [NBP2-04022]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 460 and 510 of human Target of EGR1, Member 1 using the numbering given in entry NP_079353.3 (GeneID 114034).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TOE1 Antibody - BSA Free (NBP2-04022) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TOE1 Antibody - BSA Free
Immunoprecipitation: TOE1 Antibody [NBP2-04022]
Immunoprecipitation: TOE1 Antibody [NBP2-04022] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TOE1 antibody used for IP at 6 ug/mg lysate. TOE1 was also immunoprecipitated by rabbit anti-TOE1 antibody , which recognizes a downstream epitope. For blotting immunoprecipitated TOE1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: TOE1 Antibody [NBP2-04022]](https://resources.bio-techne.com/images/products/TOE1-Antibody-Immunoprecipitation-NBP2-04022-img0002.jpg "Immunoprecipitation: TOE1 Antibody [NBP2-04022]")
Immunoprecipitation: TOE1 Antibody [NBP2-04022]
Immunoprecipitation: TOE1 Antibody [NBP2-04022] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TOE1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TOE1 was also immunoprecipitated by rabbit anti-TOE1 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).![Immunoprecipitation: TOE1 Antibody [NBP2-04022]](https://resources.bio-techne.com/images/products/TOE1-Antibody-Immunoprecipitation-NBP2-04022-img0003.jpg "Immunoprecipitation: TOE1 Antibody [NBP2-04022]")
Immunoprecipitation: TOE1 Antibody [NBP2-04022]
Immunoprecipitation: TOE1 Antibody [NBP2-04022] - Detection of human TOE1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TOE1 antibody NBP2-04022 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TOE1 was also immunoprecipitated by another rabbit anti-TOE1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for TOE1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 mcg/mg
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TOE1
Alternate Names
FLJ13949, target of EGR1 protein 1, target of EGR1, member 1 (nuclear)
Entrez Gene IDs
NP_079353.3 (Human)
Gene Symbol
TOE1
Additional TOE1 Products
Product Documents for TOE1 Antibody - BSA Free
Product Specific Notices for TOE1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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