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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 953730

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Partial recombinant human CD28H protein (amino acids 23-150) [UniProt Q96BF3]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse TMIGD2 Antibody (953730) - Azide and BSA Free (MAB83161) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TMIGD2 Antibody (953730) - Azide and BSA Free

Immunohistochemistry-Paraffin: TMIGD2 Antibody (953730) - Azide and BSA Free [MAB83161]

Immunohistochemistry-Paraffin: TMIGD2 Antibody (953730) - Azide and BSA Free [MAB83161]

Immunohistochemistry-Paraffin: TMIGD2 Antibody (953730) - Azide Free [MAB83161] - TMIGD2 was detected in immersion fixed paraffin-embedded sections of human thymus using tmigd2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cancer cells.
Flow Cytometry: TMIGD2 Antibody (953730) - Azide and BSA Free [MAB83161]

Flow Cytometry: TMIGD2 Antibody (953730) - Azide and BSA Free [MAB83161]

Flow Cytometry: TMIGD2 Antibody (953730) - Azide Free [MAB83161] - Detection of TMIGD2 in HEK293 Human Cell Line Transfected with Human TMIGD2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TMIGD2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TMIGD2 Monoclonal Antibody (Catalog # MAB83161) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Applications for TMIGD2 Antibody (953730) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that is constitutively expressed on naive T and NK cells. The interaction of CD28H with B7-H7 induces effector and memory T cell proliferation and activation. CD28H also regulates epithelial cell morphology, focal adhesion contact formation, and cell migration as well as angiogenesis. It interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Alternate Names

CD28H, IGPR-1, IGPR1

Gene Symbol

TMIGD2

Additional TMIGD2/CD28H Products

Product Documents for TMIGD2 Antibody (953730) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TMIGD2 Antibody (953730) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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