Thrombospondin-1 Antibody (A6.1)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2059
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Block/Neutralize, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, PCR, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # A6.1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Localization
Secretory granules, Golgi complex, endoplasmic reticulum
Specificity
This is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Thrombospondin-1 Antibody (A6.1)
Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]
Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-NB100-2059-img0015.jpgImmunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Staining in human placenta.Applications for Thrombospondin-1 Antibody (A6.1)
Application
Recommended Usage
Immunohistochemistry
1:25-1:50
Immunohistochemistry-Paraffin
1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Blocking/Neutralizing reported in scientific literature (PMID: 19509293). Use in Western blot reported in reported in scientific literature (PMID: 27447109). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24971453).
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Thrombospondin-1
Alternate Names
THBS1, Thrombospondin1, TSP-1
Gene Symbol
THBS1
UniProt
Additional Thrombospondin-1 Products
Product Documents for Thrombospondin-1 Antibody (A6.1)
Product Specific Notices for Thrombospondin-1 Antibody (A6.1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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