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Thrombospondin-1 Antibody (A6.1)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2059

Novus Biologicals, part of Bio-Techne
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NB100-2059
NB100-2059SS

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Block/Neutralize, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, PCR, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A6.1

Concentration

0.2 mg/ml

Product Specifications

Immunogen

BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Localization

Secretory granules, Golgi complex, endoplasmic reticulum

Specificity

This is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Thrombospondin-1 Antibody (A6.1)

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-NB100-2059-img0015.jpg
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Staining in human placenta.
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0013.jpg

Applications for Thrombospondin-1 Antibody (A6.1)

Application
Recommended Usage

Immunohistochemistry

1:25-1:50

Immunohistochemistry-Paraffin

1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Blocking/Neutralizing reported in scientific literature (PMID: 19509293). Use in Western blot reported in reported in scientific literature (PMID: 27447109). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24971453).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Thrombospondin-1

Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human); 56229 (Mouse); 364630 (Rat)

Gene Symbol

THBS1

UniProt

Additional Thrombospondin-1 Products

Product Documents for Thrombospondin-1 Antibody (A6.1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thrombospondin-1 Antibody (A6.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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