TFIIE beta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87931

Novus Biologicals, part of Bio-Techne
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NBP1-87931
NBP1-87931-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFIIE beta Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TFIIE beta Antibody - BSA Free (NBP1-87931) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-TFIIE beta Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TFIIE beta Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TFIIE beta Antibody [NBP1-87931]

Immunocytochemistry/ Immunofluorescence: TFIIE beta Antibody [NBP1-87931]

Immunocytochemistry/Immunofluorescence: TFIIE beta Antibody [NBP1-87931] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TFIIE beta Antibody [NBP1-87931]

Immunohistochemistry-Paraffin: TFIIE beta Antibody [NBP1-87931]

Immunohistochemistry-Paraffin: TFIIE beta Antibody [NBP1-87931] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
TFIIE beta Antibody - BSA Free Western Blot: TFIIE beta Antibody - BSA Free [NBP1-87931]

Western Blot: TFIIE beta Antibody - BSA Free [NBP1-87931]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
View 1 more images

Applications for TFIIE beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 26996949). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFIIE beta

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Alternate Names

FE, General transcription factor IIE subunit 2, general transcription factor IIE, polypeptide 2 (beta subunit, 34kD), general transcription factor IIE, polypeptide 2, beta 34kDa, TF2E2TFIIE-beta, TFIIE-B, transcription initiation factor IIE subunit beta

Gene Symbol

GTF2E2

Additional TFIIE beta Products

Product Documents for TFIIE beta Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFIIE beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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