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TET3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20602

Novus Biologicals, part of Bio-Techne
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NBP2-20602

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Xenopus

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34246869)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

179 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TET3 Antibody (NBP2-20602) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF, IP and ChIP. Anti-TET3 Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TET3 Antibody

Western Blot: TET3 Antibody [NBP2-20602]

Western Blot: TET3 Antibody [NBP2-20602]

TET3-Antibody-Western-Blot-NBP2-20602-img0013.jpg
Immunoprecipitation: TET3 Antibody [NBP2-20602]

Immunoprecipitation: TET3 Antibody [NBP2-20602]

Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
TET3 Antibody

Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] -

Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, & Tet3. a, b Both human (h) & mouse (m) BMMSCs expressed Tet1, Tet2, & Tet3, as assessed by western blotting (a) & qPCR (b). c Immunocytofluorescent staining showed that CD146-positive BMMSCs expressed Tet1, Tet2, & Tet3. Scale bar, 50 μm. Results are from three independent experiments. ***p <0.001; p values calculated using two-tailed Student's t test (mean ± SD) Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-04464-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TET3 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Flow Cytometry

Reported in scientific literature (PMID:34246869)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:500-1:1000

Western Blot

1:1000-1:10000

Reviewed Applications

Read 1 review rated 4 using NBP2-20602 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TET3

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM]

Alternate Names

EC 1.14.11.n2, hCG_40738, KIAA0401, methylcytosine dioxygenase TET3, MGC22014, probable methylcytosine dioxygenase TET3, tet oncogene family member 3

Gene Symbol

TET3

UniProt

Additional TET3 Products

Product Documents for TET3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TET3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.