Testosterone Antibody (4E1G2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11603
![Testosterone Antibody (4E1G2) - Azide and BSA Free Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) - Azide and BSA Free [NBP3-11603] -](https://resources.bio-techne.com/images/products/nbp3-11603_mouse-monoclonal-testosterone-antibody-4e1g2-azide-and-bsa-free-24120258195517.jpg "Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) - Azide and BSA Free [NBP3-11603] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Radioimmunoassay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4E1G2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Testosterone 3 CMO conjugated to BSA
Localization
Secreted and cytoplasmic
Specificity
This monoclonal antibody is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
288.42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45187).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Testosterone Antibody (4E1G2) - Azide and BSA Free
Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) - Azide and BSA Free [NBP3-11603] -
Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) - Azide and BSA Free [NBP3-11603] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Testosterone monoclonal antibody (4E1G2).Applications for Testosterone Antibody (4E1G2) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10-1:500
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Testosterone
Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.
Alternate Names
17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone
Additional Testosterone Products
Product Documents for Testosterone Antibody (4E1G2) - Azide and BSA Free
Product Specific Notices for Testosterone Antibody (4E1G2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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