Testosterone Antibody (4E1G2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45187

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Radioimmunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E1G2

Concentration

0.2 mg/ml

View all Testosterone Antibodies »

Product Specifications

Immunogen

Testosterone 3 CMO conjugated to BSA

Localization

Secreted and cytoplasmic

Specificity

This monoclonal antibody is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

288.42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11603)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Testosterone Antibody (4E1G2)

Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187]

Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187]

Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Testosterone monoclonal antibody (4E1G2).

Applications for Testosterone Antibody (4E1G2)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Testosterone

Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.

Alternate Names

17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone

Additional Testosterone Products

Product Documents for Testosterone Antibody (4E1G2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Testosterone Antibody (4E1G2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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