TBR1 Antibody (1U1K5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15769
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1U1K5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBR1 (Q16650). MQLEHCLSPSIMLSKKFLNVSSSYPHSGGSELVLHDHPIISTTDNLERSSPLKKITRGMTNQSDTDNFPDSKDSPGDVQRSKLSPVLDGVSELRHSFDGS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TBR1 Antibody (1U1K5) (NBP3-15769) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TBR1 Antibody (1U1K5)
![Western Blot: TBR1 Antibody (1U1K5) [NBP3-15769]](https://resources.bio-techne.com/images/products/TBR1-Antibody-1U1K5-Western-Blot-NBP3-15769-img0002.jpg "Western Blot: TBR1 Antibody (1U1K5) [NBP3-15769]")
Western Blot: TBR1 Antibody (1U1K5) [NBP3-15769]
Western Blot: TBR1 Antibody (1U1K5) [NBP3-15769] - Western blot analysis of extracts of various cell lines, using TBR1 Rabbit mAb (NBP3-15769) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: TBR1 Antibody (1U1K5) [NBP3-15769] -
Immunocytochemistry/ Immunofluorescence: TBR1 Antibody (1U1K5) [NBP3-15769] - Confocal imaging of paraffin-embedded Mouse brain using TBR1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.Applications for TBR1 Antibody (1U1K5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TBR1
Alternate Names
MGC141978, T-box brain protein 1, T-box, brain, 1, TBR-1, T-brain-1, TES-56
Gene Symbol
TBR1
Additional TBR1 Products
Product Documents for TBR1 Antibody (1U1K5)
Product Specific Notices for TBR1 Antibody (1U1K5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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