Tau Antibody - BSA Free

Western Blot: Tau Antibody [NB100-82247]

Western Blot: Tau Antibody [NB100-82247] - Analysis of extracts from mouse brain tissue.

Immunohistochemistry-Paraffin: Tau Antibody [NB100-82247] -

Immunohistochemistry-Paraffin: Tau Antibody [NB100-82247] - Analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using NB100-82247

Western Blot: Tau Antibody - BSA Free [NB100-82247] -

TIR-MAPT RNA expression. a Schematic representation of the MAPT gene and MAPT and TIR-MAPT mRNAs generated from it. A, C, D and E represent the hybridization sites of the primers designed for semi-quantitative PCR employed for the PCRs in b (Supplementary Table 2, online resource). The fragment of intron 12 that would remain upon retention is represented with colored stripes. b Representative images of agarose gels showing of semi-quantitative PCR results using total or cytoplasmic-enriched RNA of SH-SY5Y cells. Results showed the existence of RNA species from exon 11 to intron 12 where intron 11 was spliced out (PCR 5 and 6). Controls of the addition (RT+) or no addition of retrotranscriptase (RT−) were included. PCR 9 shows MAPT in which both intron 11 and 12 are spliced out. Detailed information of all semi-quantitative PCR combinations and amplicon sizes is provided in Supplementary Table 5, online resource. c Schematic representation of the MAPT gene including the hybridization sites of the oligos used for quantitative PCR. dTIR-MAPT RNA levels by qPCR in cytoplasmic-enriched fraction or whole extracts (total) of SH-SY5Y cells. e Comparison of TIR-MAPT level in SH-SY5Y cells and hippocampus and frontal lateral cortex of human brain. Graphs show means and SE of technical triplicates. f Percentage of brain samples having expression of MAPT or TIR-MAPT genes. Data are shown for three different regions within brains (see Supplementary Table 3, online resource). g Scatter dot blot of expression values of MAPT and TIR-MAPT genes in brain regions having TPM (transcripts per kilobase million) > 0. Number of samples f, g: cortex = 122; frontal cortex = 113; hippocampus = 98. Graphs represent mean and SEM. p values were calculated using a T test, ****p value ≤ 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33934221), licensed under a CC-BY license. Not internally tested by Novus Biologicals.