STEP Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03693
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 190-307 of human STEP (NP_116170.3). SFTYSEWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit STEP Antibody - Azide and BSA Free (NBP3-03693) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for STEP Antibody - Azide and BSA Free
![Western Blot: STEP AntibodyAzide and BSA Free [NBP3-03693]](https://resources.bio-techne.com/images/products/STEP-Antibody-Western-Blot-NBP3-03693-img0001.jpg "Western Blot: STEP AntibodyAzide and BSA Free [NBP3-03693]")
Western Blot: STEP AntibodyAzide and BSA Free [NBP3-03693]
Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] -
Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of Neuro-2a cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] -
Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of SH-SY5Y cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for STEP Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: STEP
Long Name
Tyrosine-protein phosphatase non-receptor type 5
Alternate Names
PTPN5
Gene Symbol
PTPN5
Additional STEP Products
Product Documents for STEP Antibody - Azide and BSA Free
Product Specific Notices for STEP Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...