Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # J2D2

Format

BSA Free

Concentration

1.0 mg/ml

View all STEAP1 Antibodies »

Product Specifications

Immunogen

Recombinant human STEAP1 (1-70aa) purified from E. coli (NP_036581).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for STEAP1 Antibody (J2D2) - BSA Free

Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunocytochemistry/Immunofluorescence: STEAP1 Antibody (J2D2) [NBP1-07094] - staining of human LNCap cell colony with monoclonalanti-human STEAP1 antibody (J2D2) .
Immunohistochemistry-Paraffin: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunohistochemistry-Paraffin: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunohistochemistry-Paraffin: STEAP1 Antibody (J2D2) [NBP1-07094] - Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Human breast cancer tissue (left) and prostate hyperplasia tissue (right).
STEAP1 Antibody (J2D2) - BSA Free

Western Blot: STEAP1 Antibody (J2D2) [NBP1-07094] -

Western Blot: STEAP1 Antibody (J2D2) [NBP1-07094] - The cell lysates (30ug) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-07094 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: LNcap cell lysate, Lane 2: Mouse Testis Tissue lysate

Applications for STEAP1 Antibody (J2D2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50SDS-PAGE 1:100-1:2000

Western Blot

1:1000-1:2000
Application Notes
For IHC-P: Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STEAP1

Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy. Clone J2D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STEAP1 protein.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Alternate Names

PRSS24

Entrez Gene IDs

26872 (Human)

Gene Symbol

STEAP1

UniProt

NP_036581

Additional STEAP1 Products

Product Documents for STEAP1 Antibody (J2D2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for STEAP1 Antibody (J2D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.