STAT3 Antibody (BLR098G)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14691
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR098G
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
between 175 and 225
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for STAT3 Antibody (BLR098G)
![Immunoprecipitation: STAT3 Antibody (BLR098G) [NBP3-14691]](https://resources.bio-techne.com/images/products/STAT3-Antibody-Immunoprecipitation-NBP3-14691-img0001.jpg "Immunoprecipitation: STAT3 Antibody (BLR098G) [NBP3-14691]")
Immunoprecipitation: STAT3 Antibody (BLR098G) [NBP3-14691]
Immunoprecipitation: STAT3 Antibody [NBP3-14691] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from NCI-H226 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiSTAT3 recombinant monoclonal antibody(A700-098 lot 1) used for IP at 6 ul per reaction. STAT3was also immunoprecipitated by rabbit anti-STAT3antibody. For blotting immunoprecipitatedSTAT3, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.
STAT3 Antibody [NBP3-14691] -
Rabbit anti-STAT3 recombinant monoclonalantibody [BLR098G]. Secondary: HRPconjugated goat anti-rabbit. IgG Substrate:DAB.
STAT3 Antibody [NBP3-14691] -
Whole cell lysate (50 µg) from RKO, NCI-H226, HEK293T,GaMG, LNCaP, HeLa, Ramos, MOLT-4, and Jurkat cellsprepared using NETN lysis buffer. Antibody: Rabbit antiSTAT3 recombinant monoclonal antibody [BLR098G] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].Applications for STAT3 Antibody (BLR098G)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 to 1:500
Immunoprecipitation
6 µl/1 mg lysate
Western Blot
1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 isrecommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: STAT3
Long Name
Signal Transducer and Activator of Transcription 3
Alternate Names
Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)
Gene Symbol
STAT3
Additional STAT3 Products
Product Documents for STAT3 Antibody (BLR098G)
Product Specific Notices for STAT3 Antibody (BLR098G)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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