SSEA-4 Antibody (MC-813-70) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26644CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Canine, Chicken, Primate, Rabbit
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # MC-813-70
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human embryonal carcinoma cell line 2102Ep
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for SSEA-4 Antibody (MC-813-70) [CoraFluor™ 1]
Application
Recommended Usage
Flow (Cell Surface)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-26644. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: SSEA-4
SSEAs were originally identified by monoclonal antibodies recognizing defined carbohydrate epitopes associated with lacto- and globo-series glycolipids. They are often used as markers for stem cell differentiation. CD15/Lewis X/SSEA-1 is expressed on murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and primordial germ cells. SSEA-3 and SSEA-4 are synthesized during oogenesis and is present on oocyte, zygote, and early cleavage-stage embryo membranes. Mouse CD15/Lewis X/SSEA-1 expression decreases with differentiation as SSEA-3 and SSEA-4 expression increase. In contrast, human EC and ES cells express SSEA-3 and SSEA-4, and differentiation is accompanied by an upregulation of CD15/Lewis X/SSEA-1 and down-regulation of SSEA-3 and SSEA-4.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4
Additional SSEA-4 Products
Product Documents for SSEA-4 Antibody (MC-813-70) [CoraFluor™ 1]
Product Specific Notices for SSEA-4 Antibody (MC-813-70) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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