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SPO11 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00023626-D01P

Novus Biologicals, part of Bio-Techne
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H00023626-D01P

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SPO11 (NP_937998.1, 1 a.a. - 358 a.a.) full-length human protein. MAFAPMGPEASFFDVLDRHRESLLAALRRGGREPPTGGSRLASRFEDSVGLQMVSHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDISCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGATAVAVPSNIQGIRNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDSLIPLTKRDQMKLDSILRRPYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPNKLKFGGWI

Specificity

Reacts with SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SPO11 Antibody - Azide and BSA Free (H00023626-D01P) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SPO11 Antibody - Azide and BSA Free

Western Blot: SPO11 Antibody [H00023626-D01P]

Western Blot: SPO11 Antibody [H00023626-D01P]

Western Blot: SPO11 Antibody [H00023626-D01P] - Analysis of SPO11 expression in transfected 293T cell line by SPO11 polyclonal antibody.Lane 1: SPO11 transfected lysate(40.40 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SPO11 Antibody [H00023626-D01P]

Immunocytochemistry/ Immunofluorescence: SPO11 Antibody [H00023626-D01P]

Immunocytochemistry/Immunofluorescence: SPO11 Antibody [H00023626-D01P] - Analysis of antibody to SPO11 on HeLa cell. Antibody concentration 30 ug/ml.

Applications for SPO11 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against transfected lysate in western blot, and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Spo11

Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq]

Alternate Names

Cancer/testis antigen 35, CT35MGC39953, meiotic recombination protein SPO11, SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae), SPO11 meiotic protein covalently bound to DSB-like (S. cerevisiae), SPO11, meiotic protein covalently bound to DSB (S. cerevisiae)-like

Entrez Gene IDs

23626 (Human)

Gene Symbol

SPO11

Additional Spo11 Products

Product Documents for SPO11 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPO11 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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