Snail Antibody - BSA Free

Western Blot: Snail Antibody - BSA Free [NBP2-29626] -

microRNA‐196a (miR‐196a) induces epithelial‐to‐mesenchymal transition (EMT) and cell motility via c‐MYC protein accumulation. (A) c‐MYC mRNA levels in control cells and OE33 miR‐196a overexpressing clones. (B) Western blot of c‐MYC protein in control cells and OE33 miR‐196a overexpressing clones. (C) VCP mRNA levels in control cells and OE33 miR‐196a overexpressing clones. (D) Luciferase reporter assay in control and miR‐196a overexpressing OE33 clones. Wild type (wt) 3′ untranslated region of VCP mRNA (vcp mRNA 3′ UTR) or deletion of putative mir‐196a binding sequences ( delta196a) were cloned downstream of the luciferase (luc) gene. Luciferase constructs were then transfected in control cells or OE33 miR‐196a overexpressing clones, and luciferase activity was quantified using the Dual‐Glo Luciferase Assay System after 48 h. (E) Reversal of the mesenchymal phenotype of OE33 miR‐196a overexpressing clones after inhibition of c‐MYC activity. Cells were plated in six well plates and treated with 50 μm of 10 074‐G5 or DMSO as control for 18 h. Scale bar, 100 μm. (F) Western blot of SNAIL1 and VIMENTIN protein levels in control cells or OE33 miR‐196a overexpressing clones, and TERT mRNA levels in control cells and OE33 miR‐196a overexpressing clones upon c‐MYC inhibition (H). Cells were plated in six well plates and treated with 50 μm of 10 074‐G5 or DMSO as control for 18 h. Data are mean + standard error of the mean of three independent experiments. **P < 0.01 and ***P < 0.001 for analysis of variance (ANOVA), plus Bonferroni post‐test. ns, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40955778), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Snail Antibody - BSA Free [NBP2-29626] -

microRNA‐196a (miR‐196a) effects are mediated by TERT. (A–D) TERT siRNA interference reduces the expression of epithelial‐to‐mesenchymal transition markers (EMT) in miR‐196a overexpressing OE33 clones both at mRNA and protein levels. Cells were transfected using Lipofectamine 2000 with TERT siRNA or control siRNA (ctrl siRNA) and gene expression was analyzed after 48 h by qPCR (A–C). Protein expression in these cells was analyzed by western blot (D). (E–G) Reduction of miR‐196a‐mediated increase in the expression of EMT markers, both at mRNA and protein levels, upon TERT inhibition. Control cells and OE33 clones overexpressing miR‐196a were treated with 20 μm of BIBR1532 or DMSO as control for 18 h, and gene and protein expression were analyzed. (H) Reduction of miR‐196a‐mediated increase in cell motility upon TERT inhibition. Control cells and OE33 clones overexpressing miR‐196a were treated with 20 μm of BIBR1532 or DMSO as control for 18 h, and cell motility was analyzed by wound‐healing assay. Data are mean + standard error of the mean of three independent experiments. **P < 0.01 and ***P < 0.001 for analysis of variance (ANOVA), plus Bonferroni post‐test. ns, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40955778), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Snail Antibody - BSA Free [NBP2-29626] -

microRNA‐196a (miR‐196a) up‐regulates TERT expression, and TERT increases NF kappaB activity and produces epithelial‐to‐mesenchymal transition (EMT) in Esophageal Adenocarcinoma cells in a telomere lengthening‐independent manner. (A) Expression of TERT, the main component of the telomerase complex, by qPCR in control and OE33 mir196a overexpressing cells. (B) TERT expression in OE33 control and OE33 clones stably overexpressing TERT or a dominant negative form of TERT (TERT DN). (C) Phenotype switch in OE33 TERT and TER‐DN overexpressing clones. (D) TERT overexpression increases NF kappaB signaling activity. Control or OE33 clones stably overexpressing TERT or TERT DN were transfected with a plasmid containing 4xNF kappaB response elements controlling the expression of downstream luciferase gene. After 48 h, protein extract was obtained, and luciferase activity was quantified using the Dual‐Glo Luciferase Assay System. (E–H) qPCR and western blots of control and OE33 TERT and TER‐DN overexpressing clones showing the expression of EMT markers at both mRNA and protein levels. Scale bar, 100 μm. Data are mean + standard error of the mean of three independent experiments. **P < 0.01 and ***P < 0.001 for analysis of variance (ANOVA), plus Bonferroni post‐test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40955778), licensed under a CC-BY license. Not internally tested by Novus Biologicals.