SMNDC1 Antibody - BSA Free

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302] - Whole cell lysates from ovarian surface epithial cells (OSE), ovarian cancer cell line A2780, breast cancer cell line MCF7. Image submitted by a verified customer review.

Western Blot: SMNDC1 Antibody [NBP1-47302]

Western Blot: SMNDC1 Antibody [NBP1-47302] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMNDC1 antibody NBP1-47302 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SPF30 was also immunoprecipitated by rabbit anti-SMNDC1 antibody NBP1-47301, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Western Blot: SMNDC1 Antibody - BSA Free [NBP1-47302] -

SMNDC1 co-localizes with nuclear speckle markers.a Overview of SMNDC1’s structure with numbered truncations (for Fig. 2f), intrinsic disorder prediction plot (MetaDisorder36), AlphaFold structure prediction with Tudor domain marked in red and positions of GFP intron-tags in green. b Live images of clonal cell lines ( alphaTC1 and HAP1) with the endogenous GFP-tag in green. c Immunoblots showing expression of WT SMNDC1 and SMNDC1-GFP fusion proteins in clonal cell lines with GFP-tag in different introns. d Live (SMNDC1-GFP intron 2-3, alphaTC1) and immunofluorescence images ( alphaTC1 WT) with nuclear staining (DRAQ5™ in red/ DAPI in blue). e Live imaging (SMNDC1-GFP intron 2-3, alphaTC1) with DRAQ5™ nuclear staining showing a cell during M-phase. f Immunofluorescence images ( alphaTC1 WT) with SMNDC1 (green) and SC35 antibody (magenta), overlap of green and magenta is white. g Live imaging (SMNDC1-GFP intron 2-3, SRRM2-RFP intron 9-10, alphaTC1) with DRAQ5™ nuclear staining (blue) showing a cell during telophase. h Co-localization analyses of interphase (n = 114) and mitotic (n = 13) cells, Pearson correlation between different channels of maximum intensity projections of z-stack images. Data shown as scatter plot + median line, analyzed by two-tailed, unpaired t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37587144), licensed under a CC-BY license. Not internally tested by Novus Biologicals.