Smad2 Antibody (CL8472) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88916

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL8472

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q15796, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Smad2 Antibody (CL8472) - BSA Free

Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916]

Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916]

Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.
Western Blot: Smad2 Antibody (CL8472) [NBP2-88916]

Western Blot: Smad2 Antibody (CL8472) [NBP2-88916]

Western Blot: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human cell line U-138MG.
Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916]

Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916]

Immunocytochemistry/Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of HeLa cells using the Anti-SMAD2 monoclonal antibody, showing specific staining in the cytosol and nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for Smad2 Antibody (CL8472) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad2

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents for Smad2 Antibody (CL8472) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smad2 Antibody (CL8472) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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