SDHAF4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SDHAF4 Antibody [NBP1-86324]

Immunocytochemistry/Immunofluorescence: SDHAF4 Antibody [NBP1-86324] - Staining of human cell line A-431 shows localization to nucleus & mitochondria. Antibody staining is shown in green.

Western Blot: SDHAF4 Antibody - BSA Free [NBP1-86324]

Analysis in control (vector only transfected HEK293T lysate) and C6orf57 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Western Blot: SDHAF4 Antibody - BSA Free [NBP1-86324] -

Structure of the SDHA-AF2-AF4 complex.a Ribbon diagram of the human SDHA-AF2-AF4 complex. SDHA is shown in gray, SDHAF2 is shown in cyan, and SDHAF4 is shown in orange. The covalent FAD is shown as a stick representation with carbons yellow, oxygens red, nitrogens blue, and phosphorous orange. The isoalloxazine functional group of the FAD is positioned between the flavin-binding domain of SDHA and the C-terminus of SDHAF4. Key interactions are shown in the inset. b Orientation of SDHAF2 and SDHAF4 in the complex. SDHA is shown as ribbons and SDHAF2 and SDHAF4 are shown as space-filling. The view is rotated 70 around the x-axis as compared to the view in (a). c Interactions between the C-terminus of SDHAF4 and the SDHA active site. The position of the conserved C-terminus is stabilized by interactions between SDHAF4D107 and SDHAF4F108 and SDHA active site residues SDHAR451 and SDHAH407. d Validation of SDHAF4 binding residues using mutagenesis. SDHAF4 containing the indicated C-terminal mutations was evaluated for the ability to displace SDHAF2 from the SDHA-AF2 complex. The assembly factors that remained bound to SDHA were visualized after the separation of the reaction on an SDS-PAGE gel. Mutations involved SDHAF4D107 (SDHAF4D107A, SDHAF4D107T and SDHAF4D107N), and SDHAF4F108 (SDHAF4F108A and SDHAF4 deltaF108). The SDS-PAGE gel is representative of n = 4 independent experiments. ImageJ quantitation of SDHAF2 (teal) and SDHAF4 (brown) was used to calculate the percentage of each assembly bound to SDHA, as compared to a control (100%). This is expressed on the y-axis of each bar graph as mean values +/- SD. Bar graphs show mean values +/- SD, and statistics were calculated by paired two-tailed Student’s t-test. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-023-44563-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.