SCNN1D Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85004

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NBP1-85004
NBP1-85004-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SCNN1D Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELLDEFARENIDSLYNVNLSKGRAALSATVPRHEPPFHLDREIRLQRLSHSGSRVRVGFRLCNSTGGDCFYRGYTSGVAAVQDWYHFHYVDILALLPAAWEDSHGSQDGHFVLSCSYDGLDCQARQFRTFHHPTYGSCYTVDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCNN1D Antibody - BSA Free

SCNN1D Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: SCNN1D Antibody [NBP1-85004]

Immunocytochemistry/ Immunofluorescence: SCNN1D Antibody [NBP1-85004]

Staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.
SCNN1D Antibody - BSA Free Immunohistochemistry-Paraffin: SCNN1D Antibody [NBP1-85004]

Immunohistochemistry-Paraffin: SCNN1D Antibody [NBP1-85004]

Staining of human cerebral cortex shows weak cytoplasmic/membranous positivity in neurons.
SCNN1D Antibody - BSA Free Immunohistochemistry-Paraffin: SCNN1D Antibody [NBP1-85004]

Immunohistochemistry-Paraffin: SCNN1D Antibody [NBP1-85004]

Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.
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Applications for SCNN1D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCNN1D

SCNN1D is a subunit of the epithelial sodium channel (ENaC). ENaC has high sodium selectivity, low conductance, and amiloride sensitivity. The epithelial Na(+) channel (ENaC) regulates Na(+) homeostasis in cells and across epithelia; in the kidney, lung and colon it plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Four homologous ENaC subunits (alpha, beta, gamma, and delta) have been isolated in mammals. Combination of alpha-, beta-, and gamma-subunits or delta-, beta-, and gamma-subunits forms fully functional channels. A delta subunit can replace the alpha subunit. However, the pharmacology, sensitivity to amiloride, conductance, and ionic selectivity of the delta/beta-gamma channel are different from those of the alpha/beta-gamma channel. FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

Long Name

Amiloride-sensitive sodium channel subunit delta

Alternate Names

Delta-ENaC, Delta-NaCH, DNACH, ENaCD, SCNED

Gene Symbol

SCNN1D

Additional SCNN1D Products

Product Documents for SCNN1D Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SCNN1D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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