SARS-CoV-2 pp1ab polyprotein Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27904
Conjugate
Catalog #
Key Product Details
Species Reactivity
Virus
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
SARS-CoV-2 pp1ab polyprotein antibody was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 pp1ab polyprotein protein. The immunogen is located within 120-170 amino acids of the SARS-CoV-2 pp1ab polyprotein protein.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for SARS-CoV-2 pp1ab polyprotein Antibody - BSA Free
Western Blot: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] -
Western Blot: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein (10-418).Antibodies: SARS-CoV-2 (COVID-19) NSP16, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 u/mL and Lane 2: 2 u/mL
ELISA: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] -
ELISA: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 ProteinAntibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, . A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (10-418) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
Immunohistochemistry: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] -
Immunohistochemistry: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (0.5 u/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.Applications for SARS-CoV-2 pp1ab polyprotein Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
0.5 ug/mL
Western Blot
0.5 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 pp1ab polyprotein
Alternate Names
2019-nCoV ORF1ab polyprotein, 2019-nCoV pp1ab, 2019-nCoV Replicase polyprotein 1ab, COVID-19 ORF1ab polyprotein, COVID-19 pp1ab, COVID-19 Replicase polyprotein 1ab, Human coronavirus Replicase polyprotein 1ab, ORF1ab polyprotein, pp1ab, Replicase polyprotein 1ab, SARS-CoV-2, SARS-CoV-2 ORF1ab polyprotein, SARSCoV2 pp1ab, SARS-CoV-2 Replicase polyprotein 1ab, Severe Acute Respiratory Syndrome Coronavirus 2
Gene Symbol
ORF1ab
Additional SARS-CoV-2 pp1ab polyprotein Products
Product Documents for SARS-CoV-2 pp1ab polyprotein Antibody - BSA Free
Product Specific Notices for SARS-CoV-2 pp1ab polyprotein Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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