Salmonella Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1087

Novus Biologicals, part of Bio-Techne
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NB600-1087
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Key Product Details

Species Reactivity

Validated:

Mouse, Bacteria

Cited:

Human, Bacteria

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

4.5 mg/ml

View all Salmonella Antibodies »

Product Specifications

Immunogen

Mixture of S. enteriditis, S. typhimurium, and S. heidelburg

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27748732).

Localization

whole cell

Specificity

This is polyvalent for Salmonella "O" & "H" antigens. Immunocaptures Salmonellae. Antiserum is not absorbed and does react with related Enterobacteriaceae.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Salmonella Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
ELISA, Immunofluorescence. May be used in place of neat antiserum in almost any appropriate antibody-based technique. Immunocaptures Salmonellae. Optimal dilutions and concentrations should be determined by the end user. Purity is >95% Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 23732169)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.01 M Phosphate Buffered Saline, pH 7.2

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

4.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Salmonella

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Alternate Names

Salmonellae

Additional Salmonella Products

Product Documents for Salmonella Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Salmonella Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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