RUNX1T1/ETO Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55747
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (98%), Rat (96%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KGGGSSSSHSRQQSPVNPDPVALDAHREFLHRPASGYVPEEIWKKAE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RUNX1T1/ETO Antibody - BSA Free
Western Blot: RUNX1T1/ETO Antibody [NBP2-55747]
Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.Immunocytochemistry/ Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747]
Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.Western Blot: RUNX1T1/ETO Antibody - BSA Free [NBP2-55747] -
RUNX1T1 is highly expressed in SCLC. (A) RUNX1T1 mRNA levels in cancer cell lines based on Affymetrix data from the CCLE. SCLC is highlighted with a red arrow and NSCLC with a blue arrow. (B) Western blot of RUNX1T1 expression in eight SCLC cell lines (DMS79, H2171, H1694, SHP77, H82, H446, H69, and SW1271) and four NSCLC cell lines (A549, H1299, H1650, and PC9); beta‐actin was used as the loading control. (C) RUNX1T1 mRNA levels in tumor samples detected by RNA‐Seq comparing SCLC with other cancers (BRCA: breast cancer, CR: colorectal cancer, GBM: glioblastoma, LUAD: lung adenoma NSCLC, LUSC: lung squamous NSCLC, PRAD: prostate adenoma cancer, SKCM: skin melanoma cancer). (D) RUNX1T1 in situ hybridization examples using RNAscope on clinical tumor samples of ‘pure’ SCLC, lung adenocarcinoma and lung squamous cell carcinoma. Red dots identify RUNX1T1 mRNA signal, green dots MYC mRNA signal. Scale bar = 50 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33084222), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for RUNX1T1/ETO Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
Validated from a verified customer review
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 5 using NBP2-55747 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RUNX1T1/ETO
Alternate Names
AML1T1protein CBFA2T1, CBFA2T1Cyclin-D-related protein, CDRMGC2796, core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related, DKFZp564B213, Eight twenty one protein, ETOFLJ33145, MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related, Protein ETO, Protein MTG8, runt-related transcription factor 1; translocated to, 1 (cyclin D-related), Zinc finger MYND domain-containing protein 2, ZMYND2myeloid translocation gene on 8q22
Gene Symbol
RUNX1T1
Additional RUNX1T1/ETO Products
Product Documents for RUNX1T1/ETO Antibody - BSA Free
Product Specific Notices for RUNX1T1/ETO Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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