Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Rubicon Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 922 and 972 of human Rubicon using the numbering given in entry NP_055502.1 (GeneID 9711).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rubicon Antibody - BSA Free

Western Blot: Rubicon Antibody [NBP1-30461]

Western Blot: Rubicon Antibody [NBP1-30461]

Western Blot: Rubicon Antibody [NBP1-30461] - Detection of Human Rubicon by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Rubicon Antibody [NBP1-30461]

Immunoprecipitation: Rubicon Antibody [NBP1-30461]

Immunoprecipitation: Rubicon Antibody [NBP1-30461] - Detection of human Rubicon by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 (lot NBP1-30461-2) used for IP at 6 ug per reaction. Rubicon was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30461-1) and another rabbit anti-Rubicon antibody. For blotting immunoprecipitated Rubicon, NBP1-30461 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Rubicon Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rubicon

Long Name

Rubicon Autophagy Regulator

Alternate Names

KIAA0226, RUBCN

Gene Symbol

RUBCN

Additional Rubicon Products

Product Documents for Rubicon Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rubicon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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