RPTOR Antibody (9N0A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16729

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16729-100ul
NBP3-16729-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9N0A4 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RPTOR Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1236-1335 of human RPTOR (Q8N122). VRIFDPRMPESVNVLQIVKGLTALDIHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

149 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit RPTOR Antibody (9N0A4) (NBP3-16729) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RPTOR Antibody (9N0A4)

Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729]

Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729]

Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: RPTOR Antibody (9N0A4) [NBP3-16729]

Immunocytochemistry/ Immunofluorescence: RPTOR Antibody (9N0A4) [NBP3-16729]

Immunocytochemistry/Immunofluorescence: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunofluorescence analysis of NIH-3T3 cells using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729]

Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729]

Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunohistochemistry of paraffin-embedded mouse kidney using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for RPTOR Antibody (9N0A4)

Application
Recommended Usage

ELISA

,Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Simple Western

1:20 - 1:250

Western Blot

1:500 - 1:2000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse brain lysate, separated by Size, antibody dilution of 1;20, 1:100, 1:250, apparent MW was 152 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPTOR

Raptor (regulatory associated protein of TOR) is a conserved 150 kDa adaptor protein composed of a unique conserved region, three HEAT repeats and seven WD-40 repeats (1). Raptor recruits mTOR substrates S6K1 (p70 S6 kinase) and 4E-BP1, and regulates mTOR kinase activity (2-3). Even though Raptor is a positive regulator of mTOR, it has been shown that under nutrient deprivation, the Raptor-mTOR association is stabilized in a manner that inhibits mTOR kinase activity (1). Raptor association with mTOR is required for efficient S6K1 and 4E0BP1 phosphorylation (4).

Long Name

Regulatory Associated Protein of mTOR

Alternate Names

KIAA1303, Raptor

Gene Symbol

RPTOR

Additional RPTOR Products

Product Documents for RPTOR Antibody (9N0A4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RPTOR Antibody (9N0A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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