RNF149 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83440
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LLLHTVKHGEKGIDVDAENCAVCIENFKVKDIIRILPCKHIFHRICIDPWLLDHRTCPMCKLDVIKALGYWGEPGDVQEMPAPESPPGRDPAANLSLALPDDDGSDESSPPSASPAESEPQCDPSFKGDAGENT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RNF149 Antibody - BSA Free (NBP1-83440) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RNF149 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: RNF149 Antibody [NBP1-83440]](https://resources.bio-techne.com/images/products/RNF149-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83440-img0006.jpg "Immunocytochemistry/ Immunofluorescence: RNF149 Antibody [NBP1-83440]")
Immunocytochemistry/ Immunofluorescence: RNF149 Antibody [NBP1-83440]
Immunocytochemistry/Immunofluorescence: RNF149 Antibody [NBP1-83440] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.![Immunohistochemistry-Paraffin: RNF149 Antibody [NBP1-83440]](https://resources.bio-techne.com/images/products/RNF149-Antibody-Immunohistochemistry-Paraffin-NBP1-83440-img0002.jpg "Immunohistochemistry-Paraffin: RNF149 Antibody [NBP1-83440]")
Immunohistochemistry-Paraffin: RNF149 Antibody [NBP1-83440]
Immunohistochemistry-Paraffin: RNF149 Antibody [NBP1-83440] - Staining of human small intestine shows distinct membranous positivity in glandular cells.![RNF149 Antibody - BSA Free Western Blot: RNF149 Antibody - BSA Free [NBP1-83440]](https://resources.bio-techne.com/images/products/nbp1-83440_rabbit-polyclonal-rnf149-antibody-8420251775434.jpg "Western Blot: RNF149 Antibody - BSA Free [NBP1-83440]")
Western Blot: RNF149 Antibody - BSA Free [NBP1-83440]
Analysis in control (vector only transfected HEK293T lysate) and RNF149 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Applications for RNF149 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF149
Alternate Names
DNA polymerase-transactivated protein 2, DNAPTP2, E3 ubiquitin-protein ligase RNF149, EC 6.3.2.-, ring finger protein 149FLJ90504
Gene Symbol
RNF149
Additional RNF149 Products
Product Documents for RNF149 Antibody - BSA Free
Product Specific Notices for RNF149 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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