RNF12 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89571
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RNF12 Antibody - BSA Free (NBP1-89571) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RNF12 Antibody - BSA Free
![RNF12 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: RNF12 Antibody - BSA Free [NBP1-89571]](https://resources.bio-techne.com/images/products/nbp1-89571_rabbit-polyclonal-rnf12-antibody-1552025851511.jpg "Immunocytochemistry/ Immunofluorescence: RNF12 Antibody - BSA Free [NBP1-89571]")
Immunocytochemistry/ Immunofluorescence: RNF12 Antibody - BSA Free [NBP1-89571]
Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.![RNF12 Antibody - BSA Free Immunohistochemistry-Paraffin: RNF12 Antibody - BSA Free [NBP1-89571]](https://resources.bio-techne.com/images/products/nbp1-89571_rabbit-polyclonal-rnf12-antibody-1552025830336.jpg "Immunohistochemistry-Paraffin: RNF12 Antibody - BSA Free [NBP1-89571]")
Immunohistochemistry-Paraffin: RNF12 Antibody - BSA Free [NBP1-89571]
Staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.Applications for RNF12 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF12
Alternate Names
DKFZp686N06224, E3 ubiquitin-protein ligase RLIM, E3 ubiquitin-protein ligase RNF12, EC 6.3.2, EC 6.3.2.-, FLJ25923, FLJ41093, LIM domain-interacting RING finger protein, MGC15161, NY-REN-43, Renal carcinoma antigen NY-REN-43, RING finger LIM domain-binding protein, RING finger protein 12FLJ42887, ring finger protein, LIM domain interacting, ring zinc finger LIM domain binding protein, ring zinc finger protein NY-REN-43antigen, R-LIM, RNF12FLJ45986
Gene Symbol
RLIM
Additional RNF12 Products
Product Documents for RNF12 Antibody - BSA Free
Product Specific Notices for RNF12 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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