RIPX Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89614

Novus Biologicals, part of Bio-Techne
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NBP1-89614-25ul
NBP1-89614
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RIPX Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQRLRQAERSRQSAELDNRLFKQDFGDKINSLQLEVEELTRQRNQLEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIPX Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RIPX Antibody [NBP1-89614]

Immunocytochemistry/ Immunofluorescence: RIPX Antibody [NBP1-89614]

Immunocytochemistry/Immunofluorescence: RIPX Antibody [NBP1-89614] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RIPX Antibody [NBP1-89614]

Immunohistochemistry-Paraffin: RIPX Antibody [NBP1-89614]

Immunohistochemistry-Paraffin: RIPX Antibody [NBP1-89614] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Western Blot: RIPX Antibody [NBP1-89614]

Western Blot: RIPX Antibody [NBP1-89614]

Western Blot: RIPX Antibody [NBP1-89614] - HEK 293 cells non-transfected (lane 1) and transfected with rat Rufy3 full length plasmid (lane 2). Band at 70kDa isoform 3 of Rufy3. Band at 55kDa isoform 1 of Rufy3. Image from a verified customer review.
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Applications for RIPX Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-89614 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIPX

Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes

Alternate Names

KIAA0871SINGAR1, protein RUFY3, rap2 interacting protein x, Rap2-interacting protein x, RIPX, RUN and FYVE domain containing 3, Singar, Singar1, Single axon-regulated protein, single axon-related 1

Gene Symbol

RUFY3

Additional RIPX Products

Product Documents for RIPX Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RIPX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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