Rhodopsin Antibody (HL2668)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48717
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2668
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain human of Rhodopsin. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Rhodopsin Antibody (HL2668) (NBP3-48717) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Rhodopsin Antibody (HL2668)
Western Blot: Rhodopsin Antibody (HL2668) [NBP3-48717] -
Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Flow Cytometry: Rhodopsin Antibody (HL2668) [NBP3-48717] -
Rhodopsin antibody [HL2668] (NBP3-48717) detects Rhodopsin protein by flow cytometry analysis.Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4�C for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:50 or a Rabbit monoclonal IgG isotype control at 4�C for 1 hour.
Western Blot: Rhodopsin Antibody (HL2668) [NBP3-48717] -
Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for Rhodopsin Antibody (HL2668)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rhodopsin
Long Name
Rhodopsin
Alternate Names
OPN2, Opsin-2, RHO
Gene Symbol
RHO
Additional Rhodopsin Products
Product Documents for Rhodopsin Antibody (HL2668)
Product Specific Notices for Rhodopsin Antibody (HL2668)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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